Medical News Blog Information

Merry Xmas and Happy Holidays 2013

Hi All,

Just a quick wish from me to you for a Happy Xmas time.

If you are lucky enough to have been born into a wealthy country, enjoy your good fortunes and have a very festive and well-fed time with friends and family over the next few days. 

But please also spare a thought for the many more people on the planet who have a much harder road to travel each and every day. Perhaps add substance to that thought with a last minute present in the name of some of your loved ones, gifted to a charity that can help. This year I am supporting  UNICEF via their excellent range of charity gifts. Polio vaccination kits will be in a couple of Xmas stockings this year!

All the very best for a safe, happy, prosperous (well-funded) 2014.

Ian 
Editor-in-chief,
Virology Down Under

Middle East respiratory syndrome coronavirus (MERS-CoV): camels, camels, camels!

Two studies in Eurosurveillance, an editorial note, A Lancet Infectious diseases report and a comment point 2 hairy toes toward camels as a harbour and source in some capacity, for MERS-CoV, or MERS-CoV-very-like, infections ticking over around the Arabian peninsula. All in the space of a week!

First up, Hemida and colleagues from Saudi Universities, China and the United States describe the search for neutralizing antibodies in animals in a 12-Dec Eurosurveillance article. Great to see Saudi Uni researchers involved. I've mentioned this virus neutralization assay and its intent before. This new study builds on that from Perera and colleagues who looked at camels and some other animals.

Some major findings from this study include:

  • Dromedary camels (n=310), sheep (n=100), goats (n=45), cattle (n=50) and chickens (n=240) from MERS-CoV hotspots in Saudi Arabia (Riyadh and Al Ahsa) were tested with the pseudoparticle neutralization (ppNT) test
  • 280 camel sera (90% of camel sera) were positive using the MERS-CoV ppNT test. No other animal sera reacted in this test
  • 96% of camels had MERS-CoV (or a close relative)-reactive antibodies by 1-year of age; two-thirds of camels that were younger than 1-year of age reacted, which suggests acquisition of these infections accrues rapidly during that 1st year, or maternal antibodies remain in the offspring
  • 54 randomly selected camel sera (18% of all camel sera) were diluted out and tested using ppNT and a standard MERS-CoV microneutralization test (MNT). High levels of calf antibody specific to BCoV did not block MERS-CoV infection nonetheless there were some similar titres to both viruses in some of the camel sera. A =4-fold higher amount of antibody reactivity towards 1 virus compared to the other defined which was the most likely virus reacting. It's possible (likely?) that camels have "seen" (been infected by) both viruses or similar viruses at some time. Some of the subset of camel sera had high levels of antibody only to MERS-CoV (or a close relative)
  • Cows did not have any sign of MERS-CoV-reacting antibodies in their sera; they did have BCoV reactivity though
  • It was not stated whether the camels were ill or healthy at sampling
So other animals were not neutralising-antibody positive but young Saudi camels, like Omani, Spanish [retired to the Canary islands] and Egyptian camels before them, had acquired and reacted to infection by MERS-CoV (or a close relative) according to these validated antibody-detection tests. Regular sampling of an animal cohort is one suggested future direction.

Secondly we have Reusken and colleagues from the Netherlands, Jordan and Germany look at animals from the first known site to harbour MERS-CoV infections in April 2012 at a hospital in Zarqa city in Jordan. This was published online 12-Dec in the same issue of Eurosurveillance. Just fyi, Prof Marion Koopmans is senior author on this study and on the study below.

Some of the key points include...
  • Sera from 3-14-month old dromedary camels (n=11), goats (n=150), sheep (n=126) and cows (n=91) were tested by an antibody microarray method, used previously by this group, and the results confirmed by identifying antibodies with the ability to neutralize MERS-CoV infection.
  • The lower levels of antibody than seen in an earlier study may reflect leftover maternal antibody protection, although the authors note than maternal camel antibodies wane within 2-months of birth and that adult camels had higher levels of antibodies
  • This study cited a reference noting that apart from cows, camels, goats and sheep are major sources of meat and milk in the region, ~1 sheep/pilgrim or ~1 camel/7 pilgrims is slaughtered in Saudi Arabia for the Hajj which equates to ~3,000,000 animals!
  • 11/11 camel and 6/126 sheep sera had antibodies that reacted with MERS-CoV but, in additional testing, the sheep sera were not able to neutralize infection by MERS-CoV
  • 23/91 cows and 128/150 goat sera reacted with the human CoV, OC43 (antigenically related to BCoV); no sera reacted with SARS-CoV
  • A broadly reactive CoV, or "pancoronavirus", PCR method was used to screen camel faeces; 3 BCoV sequences were obtained, but no sign of MERS-CoV RNA in the faeces hinting that there was not an active infection at the time of sampling. This last point assumes that MERS-CoV is excreted from the camel gut during/after an acute infection. The next study may not support that assumption.
  • It was not stated whether the camels were ill or healthy at sampling
Add young camels from Jordan to those from Saudi Arabia, Oman and retired Spaniard animals as possibly having been infected by MERS-CoV (or a...you know, similar thing) or at least having antibody acquired from their mothers. 11/11 POS may yield some more data to narrow down the age of acquisition; 3-months and seropositive could suggest MERS-CoV acquisition at or very close to birth, or simply remaining protective maternal antibody. Perhaps camel farms and farmers should be a next stop for detailed testing. 

In an Editorial note, the Eurosurveillance Editors note that these data do not define the primary source for human acquisition is still unclear.

Thirdly we have Haagmans and colleagues from the Netherlands, Qatar and the United Kingdom describing the study of the Qatari farm camels and temporally related human infection, from which MERS-CoV was detected back in late November. This article was published online by the Lancet Infectious Diseases (17-Dec).

Some key findings here include...


  • The article's introduction suggests that the genetic diversity of human MERS-CoV viruses determined to date is the result of multiple zoonotic acquisitions 
  • This study started with a 61-year-old Qatari male (61M; FT#144) farm owner who had not travelled outside Qatar and his 23-year-old male (23M; FT#150) employee
  • 61M was RT-PCR POS (upE assay) on a sputum sample (collected Oct-13) and 23M on a throat swab (collected Oct-17) and subgenomic (ORF1b and nucleocapsid [N]) sequencing at the Public Health England confirmed the detection to be MERS-CoV
  • MERS-CoV genomic sequences from the 2 human cases were placed on GenBank and called Qatar_3_2013 [61M] and Qatar_4_2013 [23M] as were camel sequences from the subsequent experiments
  • Sera, rectal swabs and flocked nasal swabs were collected from all of the farm's 14 camels as well as 5 stool samples from 3 cages, by a team wearing personal protective equipment. Samples were shipped to the Netherlands for upE, N and ORF1a RT-PCR testing
  • Vero and Huh-7 cells were inoculated with swabs that had been added to viral transport medium onsite. A single culture from Camel#7 was upE RT-PCR positive at day-4 after inoculation but no culture yielded infectious virus
  • 5/14 camel nose swabs were MERS-CoV PCR positive using upE, N and ORF1a assays
  • Sequencing of a fragment of Spike gene yielded 100% identity with other Saudi MERS-CoV sequences; sequence only differing by 1 base from the original isolate, MERS-CoV/EMC.
  • All camel sera were antibody positive using an immunofluorescence test on MERS-CoV/EMC-infected cells
  • There was no "direction" to the acquisition of MERS-CoV. Whether the camels infected the humans or the humans infected the camels could not be determined from this outbreak
  • The authors conclude that detailed cases histories are important to identify animal exposures. These might not otherwise be though important in a cursory question and answer of a patient, their family or contacts
This study adds very important data that indicate a recent or resolving MERS-CoV infection in camels. No positivity was found from gastrointestinal samples. Despite no isolation of infectious MERS-CoV, the detection of RNA is an acceptable surrogate for the presence of "live" virus in an animal or person (even if it could not propagate in vitro). So the camel story has some very important new chapters added in this series of studies.

In a Comment in LID, Ferguson and Verkhove note how the One health concept is exemplified by not only this publication; as it has been by the entire MERS-CoV story. The comment also notes the need for much more study, passive and active surveillance of human and animal disease/movements and better and faster reporting to link these, or any other, animals back to the cases that are spread across a very broad geographic region. They hold Haagmans and colleagues' article up as an example of how to get more answers and prevent sustained MERS-CoV transmission among humans from developing in the future.

Tracing and testing camels imported into the region from Africa for use as food may also open a new front to identify the transmission potential of MERS-CoV (or a similar beastie) in camel infections. Testing of pneumonia causes at these other sites both for virus and antibodies against virus is probably also warranted. 

As usual, new data bring new questions and so many papers in only a week makes for lots of questions. 

Little rhino...

To the tune of �I�m a little teapot�

I�m a little rhino,
Strain in doubt
Bind with my canyon
Bind without
When I�ve replicated
Just the right amount
You�ll need to get a tissue to blow me out


[alternate: exacerbate your wheezing and cough me out]

Thanks to Cassandra Faux for putting this one together back in 2007.

Anesthesia Teaching in Kigali - Life as a local medical student on rotation


I walked into an operating room two hours after inducing the patient with a resident. The patient was covered from head to toe in a green sheet and no one was in the room. My first thought was, �oh no, what happened, how did this patient die?� This was not an unreasonable question given the number of codes that occur in the ORs. But then I heard the steady chirping of the pulse ox and realized that my patient was still alive and seems to doing well under the sheets despite being alone and unmonitored in the room. I went to find the surgery resident to see what the situation was. His response was �we were hungry and it was lunch time so we covered him up and went for food.� This patient was having a craniotomy for a meningioma removal so his brain was exposed where the skull defect was. I don�t remember what my response was because of being so shell shocked. It turned out, after prying information out of the resident, that one of their surgical instruments were not sterilized yet despite the request being made a few days ago so they decided to break scrub until the equipment could be used 2 hours later. A typical example of the going-ons at CHUK operating rooms.

The past two days have been much more satisfying. I gave the medical students a lecture in anaphylaxis yesterday and have really taken on the role of being the medical student instructor in the OR. Having never had the opportunity to teach in the past, I have found it to be a very satisfying and rewarding job. These students are extremely easy to teach. They seem so starved for information and were so enthusiastic about any information that is taught to them. There is currently no real medical student curriculum and no one to teach them or guide them. I remember how terrible it was when I was a medical student feeling neglected during my rotations. It felt like no one really care about what you were doing. This is 500x worst. The reality here is that no one does care about them. They make their own schedules, they show up to the OR and to the ICU when they feel like it and leave when they feel like it. There is no accountability. At first I was shocked by their seemingly lack of motivation but then seeing what their rotation is like, I wouldn�t waste my time at the hospital either if I wasn�t learning anything standing around.

It wasn�t until now that I realized why there was such an emphasis on the painful 10 page H+P and presentations in med school. It pounded in the organizational skills that we needed to have in order to systematically think about problems, formulate plans and perform tasks. We had a set curriculum during our non-clinical years that provided us with our basic science framework and all the rotations that we had had a set curriculum that allowed to learn about the basics of each specialty. It seems though that no one has really provided them with a strong foundation in which to build their medical knowledge on. Things like intracranial pressure is not understood by the students, the idea of ventilation vs. oxygenation is foreign to them. It was with this in mind that along with my attendings, I decided to write a medical student curriculum. The challenging part was to decide how to do this in a way that would be sustainable. We decided to write four modules on topics that I felt like are the most vital for each medical student to know: airway management, fluid and blood management, pain management, basic life support and communication. Each module consisted of a transcript with a PowerPoint, simulator sessions, and discussion points that precisely provided all the information that is needed. The hope is that any anesthesiologist can pick up this transcript and be able to run this module without any difficulty. By providing this framework, we hope that it will create an easy transition to the local physicians who plan continue to this curriculum when both I and my attending are gone.

 Me with our residents and med students after our OB postpartum hemorrhage simulator case. The patient survived.

 My med students. Celebrating after surviving one of our anesthesia modules!

 
 This was a part of our airway management module with an airway simulator session at the sim center at the hospital.


 
 

Anesthesia in Kigali, Rwanda - What is the BIG Difference?


At CHUK, I had the opportunity to work with HRH faculty that have been in the country for a period of time. In speaking to them, their thoughts are that the current medical system doesn�t lack professionals with medical knowledge but the issue is that the system itself and the cultural background that it runs on is ineffective. There is no effective OR scheduling systems. Elective cases bump emergency cases. There�s no system in place to book emergent cases. Anesthetized patients are often left alone during the procedures. Most of the cases are performed by anesthesia technicians who are trained at a local technical college without any education by a physicians. The local attendings are not often involved in the OR cases despite being the ones responsible and definitely never do their own cases. The residents similarly are extra staff. They are extra hands that are not essential to the running of the OR. Their rooms are completely set up by the technicians who have sole control over the anesthesia supplies. The ICU consists of 5 beds who are constantly full. �ICU boarders� in the PACU are essentially homeless patient with no primary team and no one following them in the PACU and often end up in what is called a �slow code.� It�s a system of survival of the fittest. I had asked if this was a product of a lack of man power and resources or a systems issues and his answer was that it was that it was the system.

The PACU and pre-op area was one of the first areas where my first thought was �this was  medicine a third world setting.� Basic principles of anesthesia in CHUK(Central Hospital University in Kigali) I�m told are to make sure that I have any equipment I need available. Many of the things are not in the room so definitely do a pre-op check and do not rely on anyone else. Second, do not trust anyone else�s� preop. Patients are often seen the night before by a technician but their exams are not often reliable. Patients are not often pre-oped until they are in the OR, lying on the OR table. Communication is big issue in all aspects of care: hand offs, discussion with surgeons, PACU signouts, designation of roles and etc.

A summary of what I know so far:

Operating Rooms at CHUK:

Main OR: 6 ORs

OB: 2 ORs

About 6 surgeries are performed each day in OB. This is the only place where I've seen daily exploratory laparotomies for peritonitis from botched C-sections from the districts. I didn't even realize that could happen from C/S. A total of around 70 operations performed each week in all the operating rooms.

There is not an effective scheduling system for the rooms. Elective cases bump urgent and emergent cases frequently. There is a hand written schedule on a white board but cases often get moved around. Patients do not have identifying wrist bands or other identifiers and therefore confirmation of the right patient and right surgery is completely dependent on the nurse.

-          There is no morphine. Pain control consists of fentanyl, IV tramadol and IV Tylenol. I thought this was crazy but apparently patients don�t complain of pain much postop. Rwanda is a palliative care nightmare. There is no palliation even if you want it.

-          Patients have to pay for their surgery and buy the drugs they need for their surgeries and take it with them on the day of surgery in order for the procedure to occur.

-          Limiting factors for the OR

o   Supplies for the OR

o   Electricity

o   Water � although with water outages, the surgeons just have buckets of waters that they use to scrub

o   Staffing

o   Anesthesia machine � all of them are broken to some extent. There are only limited numbers of ventilators so if one is used by a patient in the PACU then one OR can�t run.

o   Patients who require ventilators postop who are spontaneous breathing are attached via a endotracheal tube elbow that is then connected to a wall O2 source when a ventilator not available. There is also a shortage of monitors so generally you keep your fingers crossed and hope that the patients continue to spontaneously breathe and assume that their vitals are normal�until you get called for a code.

o   Only 2 main OR anesthesia machines have capnography

o   There is now a code cart in one of the rooms after the recent failed accreditation visit. But there are not any defibrillators around.

-          Surgical safety checklist is being implemented by the HRH staff

-          ICU only has 5 bed and is constantly full. Patients cannot be discharged from the hospital until they pay their bills. For the patients that cannot afford to pay for their bill, they stay in the hospital in definitely. As a consequence, patients from the OR who needs an ICU bed are brought out to the PACU where they are monitored and suffer what is called a �slow code� until they pass away.

-          PACU, where over 10-15 patients are consistently and is managed by 2 nurses. Only some patients have constant vital sign monitoring. Ventilated patients who requires the ICU are not consistently managed and there is no physician who takes responsibility for these patients and many of them end up dying.

-          Patient are usually pre-oped the night before by anesthesia techs who do not relay the information to the day team. Patients are usually first seen by the anesthesia team when they are rolled into the OR and have been moved to the OR table. This is where the short preop assessment is made and the IV is started.

-          Anesthesia residents and anesthesia techs routinely leave the room leaving the anesthetized patient completely unattended.

-          Each OR is manned by either 2 anesthesia techs or 1 tech with a resident.

-          The tech is responsible for setting up the room, getting the airway equipment, drawing the drugs. The airway equipment is often not in the room during induction and are stored in a locked locker that only the techs have access to.

-          The techs are usually not supervised by anyone as the consultants who are supposed to be responsible are generally not around. The techs are the ones that usually train the residents initially and therefore they usually pick up their bad habits.

o   Machines often are not checked

o   Not all monitors may be on before induction

o   Suction is not on(only long soft tipped catheters are used)

o   Airway equipment often not checked, laryngoscopes not checked and not in the room, oral airway not available

o   No working ambu-bag

o   Not all drugs available

o   No real personalized anesthesia plan is developed for each patient, airway exam, preop assessments often are not performed

-          �infected� patients are operated on as the last case in the room in order to prevent contamination of subsequent patients no matter how urgent/emergent the case is � the HRH people are trying to stop this practice. For example, I have seen many instances where an elective C/S or D+C is performed before a peritonitis ex. lap. There is definitely a difference in the sense of urgency.

Overall I think the most important thing that I have experienced is that the physicians and residents often do have medical knowledge and have access to book and information. What needs improvement is systems based problems such as overall organizational skills, thinking over anesthetic plans, the implications of anesthetics and its actions on patients and etc.

Anesthesia Teaching in Kigali, Rwanda


This is my first trip to Africa. Rwanda has only been this textbook entry that I read about in class during to the horrible genocide 20 years ago. It�s hard to believe that I am actually here. I have been waiting and planning for the trip for the past year and yet I felt completely emotionally unprepared now that I am actually here. It�s finally starting to feel real now that I am actually here. First impressions as the plane was making its descent was �where are all the lights.� I was craning my neck with my face plastered against the plane window, searching for evidence of the city where I was going to spend the next month of my life but yet could only make out occasional flickers of light in the distance. I double checked the monitors and saw that we had 11 miles to go, 10, 8� Where were the lights? Slowly more lights appeared on the horizon, reinforcing the fact that I was likely approaching the right place.

My first day of work  at Central University Hospital of Kigali(CHUK) started on Monday, also known as their nonclinical academic day. I got eased into the schedule but sitting through a morning of lectures much like what we do at BWH. The hospital actually reminds me quite like the Hawaii hospitals. Low one story structures that almost look like small bunkers that are surrounded by greenways separating each unit. The operating rooms were surprisingly much bigger, more spacious and nicely warm instead of the frigid conditions that most of our operating rooms operate under. Being from Asia, it was easy for me to ignore the mold on the walls. The Glostavent is an amazing ventilator that I encountered for the first time. It operates in conditions where both electricity and/or oxygen supply is inconsistent. The most glaring difference in the ORs is the lack of a scavenging systems on the ventilators. For the non-anesthesiologists, this is the system that takes the wasted/exhaled gases from the patient and removes it to a scavenging waste system. Without it, the halothane that the patient was exhaling was being dumped into the OR and then being breathed in by us. I had no idea why I felt so exhausted every night after work until I found out that I was essentially being anesthetized on a daily basis.
 
 I saw this on the bulletin board in the OR.

 Our OR schedule, but don't worry, no one really follows the daily room schedule.

 The Pre-op holding area. This is not a place where you want to be when you are sick. There is generally staff taking care of patients here and patients are not often seen by any medical staff until they are lying on the OR bed.

 Our operating room.

 The Amazing Glostavent.

 Our sterile facemasks being hung outside to dry.
 

The saying �change has to come from within� definitely applies here and to the motto of the Human Resources for Health(HRH) physicians and to the foreign staff working here. Their goals are not to join the workforce but to provide guidance to the physicians already there. If they ever establish themselves as working staff, the thought is that the local staff would disappear and simply let them do all the work. One of the mistakes that was repeatedly emphasized to me is the enthusiasm of foreign staff to change the way things are done or practiced. This can happen with tremendous effort on the part of the initiator however things quickly fall apartment once the foreign presence leaves. Change can only be sustainable if a local staff takes responsibility for the project and carries it forward in the system and their peers. However not many local physicians seem to have the motivation to do this. This was not something that I had really considered before when I developed my interest in global health but makes so much sense now that I got to see it in person.

During my first week, it was easy to get lost and often found myself wondering how I could really contribute to training the students and residents here. It wasn�t until a few days into my time at CHUK that I started noticing how neglected the medical students were on their rotation that I decided to make them my students for the month. Rwanda is desperate for anesthesiologists. The country has about 12 MD anesthesiologists while most of the anesthetics are provided by anesthesia techs. The one of the goals of the anesthesia team for Human Resources for Health is to recruit residents. There is no current first year residents. I believe that recruitment should start with medical students. Also after graduation, medical students are required to have an intern year and then practice independently for 2 years as a general practitioner before being eligible to apply for residents. I had a month to prepare these medical students with all the basic knowledge of airway assessment, fluid and blood management and pain management that they needed to take care of patients in the district hospitals when they are the sole practitioner 2 years from now. It was a daunting task!

Twelve weeks of childcare...

To the tune of the twelve days of Xmas

In the first week of childcare,
some kids got sick with me
The lab said they might have HRV

In the second week of childcare,
some kids got sick with me,
Two had paraflu,
and a few might have had an HRV

In the third week of childcare,
some kids got sick with me,
Three had an entero
Two had paraflu
and a few had uncultivable HRV

In the fourth week of childcare,
some kids got sick with me,
Four had OC43
Three had an entero
Two had paraflu
and a few had untypeable HRVs

In the fifth week of childcare,
some kids got sick with me,
Five had MPV
Four had OC43
Three had an entero
Two had paraflu
and a few had something like an HEV

In the sixth week of childcare,
some kids got sick with me,
Six had HKU1
Five had MPV
Four had OC43
Three had an entero
Two had paraflu
and a few had newly identified HRVs

In the seventh week of childcare,
some kids got sick with me,
Seven had an adeno
Six had HKU1
Five had MPV
Four had OC43
Three had an entero
Two had paraflu
and a few had pleconaril-resistant HRVs (we think!)

In the eighth week of childcare,
some kids got sick with me,
Eight had bocavirus
Seven had an adeno
Six had HKU1
Five had MPVs
Four had OC43
Three had an entero
Two had paraflus
and a few were PCR-positive for HRV

In the ninth week of childcare,
some kids got sick with me,
Nine had RSV
Eight had bocavirus
Seven had an adeno
Six had HKU1
Five had MPV
Four had OC43
Three had an entero
Two had paraflu
and a few had antigenically distinct HRVs

In the tenth week of childcare,
some kids got sick with me,
Ten had NL63
Nine had RSV
Eight had bocavirus
Seven had an adeno
Six had HKU1
Five had MPV
Four had OC43
Three had an entero
Two had paraflu
and a few had more than a single HRV

In the eleventh week of childcare,
some kids got sick with me,
Eleven had Wuv and Kiv
Ten had NL63
Nine had RSV
Eight had bocavirus
Seven had an adeno
Six had HKU1
Five had MPV
Four had OC43
Three had an entero
Two had paraflu
and some came from a clade of HRV (which was distinct!)

In the twelfth week of childcare,
some kids got sick with me,
Twelve had IFAV
Eleven had Wuv and Kiv
Ten had NL63
Nine had RSV
Eight had bocavirus
Seven had an adeno
Six had HKU1
Five had MPV
Four had OC43
Three had an entero
Two had paraflu

and a few had what we call HRV C

Thanks to Katherine Arden and Cassandra Faux for helping me put these together back in 2008.

You're the virus I got...

To the tune of Grease/You�re the one that I want

I�ve got chills, they�re multiplyin�, and I�ve got a sore throat
Home remedies that I�m tryin�, feels like I�m dyin

Really runny nose, now I cough and ache
and the doctor says its flu
Really runny nose, now it�s in my chest
To my lab I must be true,
There�s nothing  left, nothing left for Doc to do

Chorus:
It�s a rhino I�ve got
(a tiny little rhinovirus), not the flu, Doctor
A rhino I�ve got (a tiny little rhinovirus),
not the flu, Doctor
A rhino I�ve got (a tiny little rhinovirus),
not the flu
It�s all I need (an A or C)
Oh yes indeed (yes indeed)

If you�re filled with secretions
Lungs too hard to inflate
Medicate, must aren�t worth mention, resistance you�ll make

Starting to wheeze, cause of cytokines
I need some air, maybe �roids will pull me through
Starting to wheeze, is it RSV?
I don�t have two, just an HRV that�s all
Are you sure? Well those PCRs wouldn�t lie


Chorus: (repeats out)

Thanks to Katherine Arden and Cassandra Faux for helping me put these together back in 2008.

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